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Interactive Tools & Utilities

DNA / RNA Sequence Toolkit

An all-in-one browser-based sequence analysis suite. Calculate GC content, generate reverse complements, perform 3-frame and 6-frame translations, and estimate primer melting temperatures (Tm) instantly without installing any software.

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What this tool calculates

Paste a DNA or RNA sequence to instantly get its length, GC/AT content, molecular weight, complement and reverse complement, a three-frame protein translation using the standard genetic code, and an estimated melting temperature (Tm) for primer design. All calculations run locally in your browser — no sequence data is uploaded or stored.

DNA / RNA Sequence Toolkit

Paste a nucleotide sequence to get GC content, reverse complement, three-frame translation, estimated Tm and molecular weight — all computed instantly in your browser.

DNA
⚠ Non-standard characters detected and ignored (only A, T/U, C, G, N are processed).
39 nt

Complement & reverse complement

Complement
Reverse comp.

Translation (standard genetic code)

Melting temperature (Tm) — primer estimate

All calculations run locally in your browser — no sequence data is transmitted or stored. Translation uses the standard genetic code (NCBI table 1). Tm is estimated using the Wallace rule for sequences under 14 nt and a GC-based approximation for longer sequences; for primer design in a wet lab, confirm with a nearest-neighbor thermodynamic calculator. Molecular weight is an approximation for single-stranded nucleic acid.

Editorial Team
← Dilution & Molarity Calculator FASTA Validator and Converter →

FAQ

  • How is GC content calculated?

    GC content is the percentage of guanine (G) and cytosine (C) bases in the sequence relative to its total length. It's a key indicator of DNA stability and is commonly used in PCR primer design and genome analysis.
  • What genetic code is used for translation?

    The tool uses the standard genetic code (NCBI translation table 1). Stop codons (TAA, TAG, TGA) are shown as an asterisk (*) in the translated protein sequence.
  • How accurate is the Tm (melting temperature) estimate?

    For sequences under 14 nucleotides, the tool uses the Wallace rule (4°C per G/C, 2°C per A/T). For longer sequences, it uses a GC-based approximation. Both are quick estimates suitable for initial primer screening; for precise primer design, use a nearest-neighbor thermodynamic calculator.
  • Does this tool work with both DNA and RNA sequences?

    Yes. The tool automatically detects whether you've entered DNA or RNA based on the presence of uracil (U) instead of thymine (T), and adjusts the complement and translation outputs accordingly.
  • Is my sequence data stored or sent anywhere?

    No. All calculations are performed client-side in your browser using JavaScript. No sequence is transmitted to a server or stored.